Visualize Cells And Gene Expression By Dimension Reduction

dimGenePlot3D()

Visualize cells and gene expression according to dimension reduction coordinates.

dimGenePlot3D(
    gobject,
    expression_values = c("normalized", "scaled", "custom"),
    genes = NULL,
    dim_reduction_to_use = "umap",
    dim_reduction_name = "umap",
    dim1_to_use = 1,
    dim2_to_use = 2,
    dim3_to_use = 3,
    show_NN_network = F,
    nn_network_to_use = "sNN",
    network_name = "sNN.pca",
    network_color = "lightgray",
    cluster_column = NULL,
    select_cell_groups = NULL,
    select_cells = NULL,
    show_other_cells = T,
    other_cell_color = "lightgrey",
    other_point_size = 1,
    edge_alpha = NULL,
    point_size = 2,
    genes_high_color = NULL,
    genes_mid_color = "white",
    genes_low_color = "blue",
    show_legend = T,
    show_plot = NA,
    return_plot = NA,
    save_plot = NA,
    save_param = list(),
    default_save_name = "dimGenePlot3D"
)

Arguments

gobject

giotto object

expression_values

gene expression values to use

genes

genes to show

dim_reduction_to_use

dimension reduction to use

dim_reduction_name

dimension reduction name

dim1_to_use

dimension to use on x-axis

dim2_to_use

dimension to use on y-axis

dim3_to_use

dimension to use on z-axis

show_NN_network

show underlying NN network

nn_network_to_use

type of NN network to use (kNN vs sNN)

network_name

name of NN network to use, if show_NN_network = TRUE

network_color

color of NN network

cluster_column

cluster column to select groups

select_cell_groups

select subset of cells/clusters based on cell_color parameter

select_cells

select subset of cells based on cell IDs

show_other_cells

display not selected cells

other_cell_color

color of not selected cells

other_point_size

size of not selected cells

edge_alpha

column to use for alpha of the edges

point_size

size of point (cell)

genes_high_color

color for high expression levels

genes_mid_color

color for medium expression levels

genes_low_color

color for low expression levels

show_legend

show legend

show_plot

show plots

return_plot

return ggplot object

save_plot

directly save the plot [boolean]

save_param

list of saving parameters, see showSaveParameters()

default_save_name

default save name for saving, don’t change, change save_name in save_param

Value

A ggplot.

Details

Description of parameters …

See also

Other dimension reduction gene expression visualizations: dimGenePlot2D(), dimGenePlot().